Limitations of yeast surface display in engineering proteins of high thermostability
نویسندگان
چکیده
منابع مشابه
Limitations of yeast surface display in engineering proteins of high thermostability.
Engineering proteins that can fold to unique structures remains a challenge. Protein stability has previously been engineered via the observed correlation between thermal stability and eukaryotic secretion level. To explore the limits of an expression-based approach, variants of the highly thermostable three-helix bundle protein alpha3D were studied using yeast surface display. A library of alp...
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ژورنال
عنوان ژورنال: Protein Engineering, Design and Selection
سال: 2006
ISSN: 1741-0134,1741-0126
DOI: 10.1093/protein/gzl003